Two activators of microtubule-based vesicle transport.
نویسندگان
چکیده
منابع مشابه
Two activators of microtubule-based vesicle transport
Cytoplasmic dynein purified by nucleotide dependent microtubule affinity has significant minus end-directed vesicle motor activity that decreases with each further purification step. Highly purified dynein causes membrane vesicles to bind but not move on microtubules. We exploited these observations to develop an assay for factors that, in combination with dynein, would permit minus end-directe...
متن کاملRegulation of microtubule-based transport by MAP4
Microtubule (MT)-based transport of organelles driven by the opposing MT motors kinesins and dynein is tightly regulated in cells, but the underlying molecular mechanisms remain largely unknown. Here we tested the regulation of MT transport by the ubiquitous protein MAP4 using Xenopus melanophores as an experimental system. In these cells, pigment granules (melanosomes) move along MTs to the ce...
متن کاملIntracellular transport using microtubule-based motors.
INTRODUCTION .............................................................................................................. 347 IN VITRO SYSTEMS FOR STUDYING MICROTUBULE-BASED ORGANELLE TRANSPORT .............. 349 Axonal Transport ..................................................................................................... 349 Organdie Transport in Nonneuronal Cells ......................
متن کاملBiogenesis of the crystalloid organelle in Plasmodium involves microtubule-dependent vesicle transport and assembly
Malaria parasites possess unique subcellular structures and organelles. One of these is the crystalloid, a multivesicular organelle that forms during the parasite's development in vector mosquitoes. The formation and function of these organelles remain poorly understood. A family of six conserved and modular proteins named LCCL-lectin adhesive-like proteins (LAPs), which have essential roles in...
متن کاملMicrotubule-dependent vesicle transport: modulation of channel and transporter activity in liver and kidney.
Microtubule-based vesicle transport driven by kinesin and cytoplasmic dynein motor proteins facilitates several membrane-trafficking steps including elements of endocytosis and exocytosis in many different cell types. Most early studies on the role of microtubule-dependent vesicle transport in membrane trafficking focused either on neurons or on simple cell lines. More recently, other work has ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: The Journal of Cell Biology
سال: 1991
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.115.5.1309